NovoArc‘s lipids were tested formRNA delivery with lipid nanoparticles. The uptake and the transfection efficiency of lipid nanoparticles incorporating tetraether lipids were compared to conventional lipid nanoparticle formulations. In vitro studies with three different cell lines were conducted: human skeletal myoblasts (HSkM), cancerous murine myoblast cell line (C2C12) and epithelial colon cell line (Caco2). Cells were transfected with mRNA coding for GFP. NovoArc’s formulation was able to boost the transfection efficiency significantly. Transfection efficiency inCaco2 cells showed an increase by a factor of 11.